The analysis revealed that all children and adolescents of both groups had symptoms of intoxication, intolerance of anti-tuberculosis drugs in the form of nausea, vomiting, reduced appetite, pains in the epigastric region before taking goat milk.
Goat milk was given to patients of the first group after taking anti-tuberculosis drugs (one day), young children in 100.0, adolescents in 200.0 ml for 3 months.
We studied the detoxification effect of goat's milk in patients with tuberculosis in the lungs of children and adolescents at various times (after 1 month, 2 months, 3 months).
Analysis of the obtained data showed the effectiveness of using goat milk for detoxification purposes at various stages of treatment of patients with pulmonary tuberculosis. Thus, symptoms of intoxication after 1 month of goat milk intake were eliminated in sick children and adolescents of I and II subgroups in 66.7% and 50.0% cases, respectively, after 2 months the symptoms of intoxication were stopped in 26.5% and 33.3% of patients, after 3 months of goat milk intake disappeared in 6.8% and 16.7%, respectively.
Nausea intolerance phenomena, which were observed in half of the children and adolescents of the first group, were stopped after 1 month of goat milk intake in 66.7% of patients, after 2 months in the remaining 33.3% of patients. In all cases (100.0%), patients of group I had a reduced appetite before starting milk, and after a month of goat milk, appetite increased in 80.0% of patients. Vomiting was observed before taking milk in 5 (16.7%) and stopped 1 week after taking goat milk in 2 (6.7%) children and adolescents, after 2 weeks vomiting stopped in the remaining children.
In children and adolescents from the second group, intolerance phenomena were stopped 2 months after symptomatic treatment in 60.0%, after 3 months in the remaining (40.0%) children and adolescents.
Analysis of humoral and cellular composition showed that these rates increased markedly after taking goat milk in children and adolescents of the first group, while in children and adolescents of the second group, humoral and cellular immunity remained at the same level.
Before the research, to children and teenagers of both groups conducted complex immunological blood test which included studying indicators of cellular (CD3, CD3+CD4+, CD3+CD8+, CD3+CD4+/CD3+CD8+, CD3+CD56+, CD3+CD25+, CD3+HLA-DR+) and humoral (CD19, CD3-CD25+, CD3-HLA-DR+IgM, IgA, IgG, the CEC) immunity.
The study of the quantitative composition of lymphocyte subpopulations in peripheral blood was performed on a flow cytometer Facscan from “BectonDickinson” (USA) using triple combinations of direct monoclonal antibodies (CD4/CD8/CD3, CD3/CD19, CD56/HLA-DR/CD3, CD25/CD3), Leucogate (CD45/CD14) to isolate the lymphocytic ring and isotypic control (IgG1/IgG2) of the same firm.
Serum immunoglobulins of classes M, G and A were determined by G. Mancinietal simple radial immunodiffusion. (1965). Determination of circulating immune complexes (CEC) was carried out by the method proposed by Yu.A. Grinevich and A.N. Alferov (1981) [9,11].
Before the study, 50.2% and 45.0% of children and adolescents of both groups showed a decrease in humoral and cellular immunity. At least 48.5% of patients showed activation of B-cell immune response, reflected in increased content of B-lymphocytes expressing HLA-DR, serum immunoglobulins M, A, G and low molecular weight circulating immune complexes. An increase in the concentration of low molecular weight CECs could be a prerequisite for their deposition in tissues and the development of immunopathological reactions [4,6,9,10].
After completing the course of taking goat's milk, as can be seen from Table 1, in patients with MDR TB, children and adolescents of group I CD3 reliably (p≤0,5) increased from 40.5 ± 4.46 to 46.1 ± 14.66, and in group II this indicator was preserved in the previous figures - 43.6 ± 10.42 (before), 43.8 ± 2.14 (after) (p≤0,5). The indicator CD4 significantly increased from 22.5 ± 4.15 to 27.6 ± 2.62 (p≤0,5), and in children and adolescents of the control group this indicator almost remained the same - 23.5 ± 3.74. 24.6 ± 4.33, respectively. The same pattern is observed in the analysis of CD8, Helper/suppressor indices, blood enzyme-linked.
Thus, analysis of humoral and cellular composition showed that these indicators increased markedly after taking goat milk in children and adolescents of the first group, while in children and adolescents of the second group, immunity indicators remained at the same level.